肝泡球蚴病组织中S-100^+DC(树突状细胞)、巨噬细胞及淋巴细胞的免疫组化研究

Immunohistochemical Study of S-100^+DC, Macrophage Cells and T, B Lymphocytes in Human Echinococcus multilocularis

应用免疫组化技术,对12例人肝泡状棘球蚴(Em)及6例肝海绵状血管瘤瘤旁肝组织内S-100^+DC、CD_(68)^+细胞(巨噬细胞)、OPD_4^+细胞(T辅助细胞)及CD_(20)^+细胞(B淋巴细胞)进行了定量比较研究。结果显示:(1)S-100^+DC、OPD_4^+细胞及CD_(20)^+细胞在Em组均明显高于海绵状血管瘤组(P<0.05);(2)在Em组中OPD_4^+细胞数量多于CD_(20)^+细胞数(P<0.05);(3)S-100~DC数与OPD_4^+细胞呈负相关(r_s=-0.5818,P<0.05)。提示S-100^+DC在人肝Em的免疫反应中有某些作用,其作用环节有待阐明。

We quanlified and compared the density of S-100 protein positive dentritic cells (S-100+DC), CD_(58)^+ cells, OPD_4^+ cells and CD_(20)^+ cells in the liver from 12 patients with Em and 6 patients with cavernous hemangioma by means of immunohistochemistry. The results indicated that the degree of S- 100+DC,OPD_4^+ cells and CD_(20)^+ cells is higher in Em than those in hemangioma, that OPD_4^+ cells in Em is higher than CD_(20)^+ cells. The degree of OPD_4^+ cells showed a negative relationship with S-100+DC in- filtrating (Y_s=-0.5818,P<0.05). Our data suggested that S-100+DC may play a important role in immunological response of hepatic Em disease.