肝细粒棘球蚴病肝组织细胞免疫及体液免疫反应的免疫组化定量研究

Quantitative Assessment of Lymphocytes and Its Subpopulation in the Liver of the Patients with Echinococcosis

本研究用OK系列单克隆抗体对肝包虫患者肝组织的B、T淋巴细胞及其亚群进行免疫组化染色,且对其细胞密度、比值进行定量分析。发现肝包虫患者肝组织(距病灶>5cm),外囊及毗邻肝组织中CD_3^+、CD_(19)^+、CD_4^+及CD_8^+细胞密度均较对照组显著增高(P<0.01),外囊及毗邻肝组织CD_4^+/CD_8^+细胞的比值较肝包虫肝组织及对照组均有显著降低(P<0.01)。肝包虫肝组织内CD_(19)^+细胞与CD_3^+细胞密度间无显著性差异(P>0.1)。在肝实质区的灶状坏死区内,CD_8^+细胞占优势。实验结果表明,在肝包虫感染时体液免疫及细胞免疫均增强,局部组织反应中CD_8^+细胞占优势提示细胞免疫以抑制性免疫为主,且此抑制性免疫反应有梯度变化,距包虫囊越近则免疫抑制越强,CD_4^+细胞与CD_8^+细胞密度改变可能与肝组织纤维化有关。包虫病时体液免疫与细胞免疫为两个独立的免疫系统,无相关关系。本文研究结果可为包虫病患者化疗、预后提供科学依据。

Using OKT series of monoclonal antibodies for immunostaining. We determined the cell densities of B、T lymphocytes and its subpopulations in the liver of patients with hydatidosis. The results indicate that the densities of CD_(19)^+, CD_3^+, CD_4^+ and CD_8^+ in adventitious layer and adjoining tissues of the patients were higher than that in the control, the difference was significant (P<0.01). The CD_4^+/ CD_8^+ ratio of the adventitious layer and adjacent liver tissue in these patients is significantly decreased compared to the control (P<0.01). The difference of the cell densities between the CD_(19)^+ cells and CD_3^+ cells in the patients was not significant (P> 0.1). The patients affected with echinocoecosis de- veloped a strong humoral and celular immune response and CD_8^+ ells predominate in the lesions of the liver. It showed that the immune suppression may play a major role in the specific cellular immune response and had a gradient character. Such pattern presented that closed to the larval cyst the immune suppression is stronger than those in other areas. The changes of the cell densities of CD_4^+ and CD_8^+ cells may be associated with the fibrosis of the liver. The humoral and cellular immune respense belong to two different immune systems. Our present results may be valuable for us to evaluate the clinical practice both in chemical therapy and cooperative immunotherapy of hydatidosis in human liver.