高速逆流色谱法分离纯化甘草查尔酮甲和胀果香豆素甲

Isolation and Purification of Licochalcone A and inflacoumarin A with High-speed Cunter-current Chromatography

采用高速逆流色谱法,从胀果甘草的乙醇粗提物中分离纯化甘草查尔酮甲和胀果香豆素甲。甘草查尔酮甲和胀果香豆素甲的结构用1H-NMR、13C-NMR、UV、FTIR和EI-MS鉴定。纯度用HPLC方法测定,采用归一化计算。分离采用的溶剂系统为正己烷-氯仿-甲醇-水(5:6:3:2),上相做固定相,下相做流动相,流动相流速为1.8 mL／min,甘草查尔酮甲和胀果香豆素甲的纯度分别为95.0％和98.8％。纯化采用的溶剂系统为正己烷-氯仿-甲醇-水(1.5:6:3:2),上相做固定相,下相做流动相,流动相流速为1.5 mL／min。纯化后甘草查尔酮甲和胀果香豆素甲的纯度分别为99.1％和99.6％。该方法操作简单,费用低廉,重现性好,可做为高纯度甘草查尔酮甲和胀果香豆素甲化学对照品的制备分离方法。

High speed counter-current chromatography (HSCCC) was used to seperate and purify licochalcone A and inflacoumarin A from crude ethenol extract. identifiction of structure was with 1H-NMR, 13C-NMR, UV, FTIR and EI-MS. Purity measurement was with HPLC, whose solvent system was with normal hexane-chloroform-methanol-water (5 : 6 : 3 : 2), with upper phase being stationary phase and lower mobile (1. 8ml/min). The purity degrees were 95. 0% and 98. 8% ,respectively posterior to seperation and were 99. 1% and 99. 6% posterior to purification. HSCCC is simple, economical and with good repeatibility for the purpose.