鼠伤寒杆菌外膜蛋白免疫原性初步研究

A primary study on immunogenicity of outer membrance proteine of Salmonella typhimurium

研究目的寻找鼠伤寒杆菌与免疫相关组分,探讨鼠伤寒杆菌外膜蛋白作为亚单位疫苗预防鼠伤寒杆菌感染的可能性。研究背景鼠伤寒沙门氏菌感染甚为普遍,而目前尚缺乏有效的防疫方法,制备有效疫苗是控制鼠伤寒杆菌感染和流行的重要措施。研究方法采用Schnaitman所描述的方法提取鼠伤寒杆菌OMP分别免疫小鼠和家兔后分离血清,用ELISA方法测定免疫血清抗体滴度。用小鼠作足垫肿胀试验作为测定细胞免疫指标。用免疫印迹试验分析免疫血清和鼠伤寒感染患者恢复期血清。研究结果鼠伤寒杆菌外膜蛋白能诱发小鼠和家兔产生高效价抗体,并能诱发小鼠产生明显的DTH反应,所有免疫血清均识别36KD蛋白带。结论鼠伤寒杆菌外膜蛋白具有较强的免疫原性,能诱发动物产生体液免疫和细胞免疫。36KD蛋白带可能是鼠伤寒杆菌主要免疫原。

Objective This study is to look for the im-mune element Salmonella typhimurium and re·search of its subunit vaccine pl‘otecting fromSalmonella typhimurium infection. Background The infection of Salmonellatyphimurium is very widspread,but there is 1101 el:fective methods of immunity tO protect from theinfection of S.typhimruium. Methods Rabbits and mice were immunizedwith extracted outer membrance protein(OM P)of S.typhimurium using the method described bySchnaitman CA(1 97 1).The antibody titer wasmeasured by ELISA and footpal swelling test wasdone in mice.Antiserum of 0MP in mice andconvalescent serum of patient with S.typhimurium infection was analysed with Westernblot. Results OMP of S.typhimurium have astronger immunogenicity for mice and rabitt.OMP capable of inducing delayed——typehypersensitiVity,and all of antiserum can reactionwith the protein of 36KD band. Conclusion All these result indicate that theOM P can inducing the active and positive immu—nity against the infection of S.typhimurium andthe protein band 36KD may be the position of immunogenicity.