糖肾汤对实验性糖尿病大鼠早期肾脏病变的影响

Effects of Tangshentang on renal lesions of early stage in experimental diabetic rats

目的 观察糖肾汤对实验性糖尿病大鼠早期肾脏病变的作用 ,并探讨其作用机制。方法 Wis tar大鼠 30只 ,链脲佐菌素 (STZ)腹腔内注射诱导产生糖尿病 (DM)模型 ,将成模后的大鼠随机分为模型对照组(M)、苯那普利治疗组 (B)、糖肾汤治疗组 (T) ,分别给予饮用水、苯那普利和糖肾汤治疗 ,8周后观察各组大鼠的血糖、甘油三酯、尿素氮、血肌酐、尿微量白蛋白 ,取肾组织行光镜、电镜及免疫组化。结果 糖肾汤可明显减轻DM大鼠肾小球肥大、系膜细胞增生、系膜基质增生及肾小球基底膜厚度 ,减少尿微量白蛋白的排泄 ,减轻DM大鼠肾脏形态学损害。结论 糖肾汤能改善DM大鼠的糖、脂代谢 ,对实验性DM大鼠早期肾脏病变有防治作用 ,其机制可能与抑制肾组织内转化生长因子 β1(TGF β1)

Objective To investigate the effects of Tangshentang on renal lesions of early stage in experimental diabetic rats and to explore its mechanism. Methods The rat diabetic model was induced by intraperitoneal injection of streptozotocin. The model rats were randomly divided into model control group, Benazepril group and Tangshentang group. Then physiological saline, Benazepril and Tangshentang were used to treat them respectively. After 8 weeks, the changes of serum indicators and the renal tissue in rats were observed by microscope and electron microscope. Results Tangshentang could reduce the large-vessel atherosclerosis, the thickening of glomerular basement membrane, the UAER, the damage of renal morphology. Conclusion Tangshentang can improve blood glucose and blood lipid, exerted obviously a meliorative effect in renal function and structive in diabetic rats and the important mechanism may be Tangshentang decreasing the expressions of TGF-β1 in diabetic rats.