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分子生物学技术在环境微生物多相分类中的应用 被引量：4

The Application of Molecular Biology Technology in Polyphasic Taxonomy of Environmental Microorganisms
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摘要 概述了分子生物学技术包括DNA(G+C)mol%的测定、核酸杂交、分子标记技术、蛋白质技术等在环境微生物多相分类中的应用,分析了各项技术的优缺点,介绍了最新进展和发展的方向。 The application of molecular biology techniques in polyphasic taxonomy of environmental microorganisms is summarized, which includes DNA (G+C) mol% analysis, nucleic acid hybridization, 16sr DNA sequencing, molecular marker techniques, such as RFLP, RAPD, AFLP, SSR, ISSR, etc. These techniques' advantages and disadvantages in polyphasic taxonomy of environmental microorganisms have been analyzed. The use of molecular biology techniques to environmental microorganisms research provides new understanding of microbial community. They give us a more complete picture of this area.
作者 叶亚新 黄勇 王金虎
机构地区 苏州科技学院生物系 苏州科技学院环境科学与工程系
出处 《苏州科技学院学报（自然科学版）》 CAS 2004年第4期46-53,共8页 Journal of Suzhou University of Science and Technology （Natural Science Edition）
关键词 环境微生物 多相分类 分子生物学技术 应用 environmental microorganisms polyphasic taxonomy molecular biology technology application
分类号 Q93 [生物学—微生物学]
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参考文献32

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二级参考文献25

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苏州科技学院学报（自然科学版）
2004年 第4期

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