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HPLC-UV对人组织激态释放酶的测定方法研究 被引量：1

Determination of Recombinant Human Tissue Kallikrein by Using HPLC-UV
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摘要 HPLC-UV结合的方法是一种灵敏度高、分辨率高的分离检测方法。本文利用HPLC-UV测定了人组织激肽释放酶的最大吸收波长,结果表明人组织激肽释放酶在200nm具有最大紫外吸收;并对昆虫细胞培养液中的人组织激肽释放酶进行定性检测,具有灵敏度高,所用的分析时间短等特点。 HPLC-UV is a kind of detective method with high sensitivity and high resolving power. In this paper a method for determination of recombinant human tissue kallikrein in insect cells culture by using HPLC-UV was developed. The best UV absorbance for recombinant human tissue kallikrein is 200nm.
作者 杨青 梁振 段继诚 张维冰 张玉奎
机构地区 中国科学院大连化学物理研究所国家色谱研究分析中心
出处 《光散射学报》 2004年第4期370-373,共4页 The Journal of Light Scattering
关键词 高效液相色谱 紫外检测器 人组织激态释放酶 High performance liquid chromatography UV recombinant human tissue kallikrein
分类号 O657.7 [理学—分析化学] O657.32 [理学—分析化学]
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参考文献9

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二级参考文献16

共引文献14

同被引文献10

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光散射学报
2004年 第4期

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