一种新抗冻剂DMF在淡水鱼类精液超低温保存上的应用

APPLICATION OF A NEW CRYOPROTECTANT ON CRYOPRESE RVATION OF FRESHWTER FISH SPERM

本文首次报导了一种新的抗冻剂——N-N-二甲基甲酰胺(DimethylFormamide,DMF)对淡水鱼类精液超低温冷冻保存具有明显效果,冻精的受精效果优于二甲亚砜(DMSO)。团头鲂、鲤、草鱼、鲢、鳙使用13%DMF抗冻剂,精液液氮保存3—4周,受精率分别为76%、54.03%、68.6%、37.9%和40.60%,而用13%DMSO作为抗冻剂时,则分别为69.1%、42.6%、67.6%、19.4%和37.4%。结果表明10—15%DMF作为抗冻剂,含0.5%NaCl的稀释液稀释精液,精液与稀释液按1:4比例混合冷冻;2%NaCl作为激活剂,以10倍体积激活剂稀释解冻精液进行授精为宜。

In this paper, a new kind of cryoprotectant-Dimethyl formamide (DMF) is be-ing reported that it has a significant effect on cryopreservation for freshwater fish sperm. The ferti-lizing rates of thawed sperm in blunt snout bream, common carp, grass carp, silver carp andbighead carp are 76%, 54.03%, 68.6%, 37.9% and 40.6%, respectively. Using 10-15% DMF ascryoprotectant and 0.5% NaCl in extender were available 2%NaCl as active solution and 10times volume of active solution to dilute thawed sperm during fertilization were necessary.