团头鲂乳酸脱氢酶与苹果酸脱氢酶组织特异性的激光扫描分析

DENSITOMETRIC SCANNING ANALYSIS ON TISSUE SPECIFIC ISOZYMES OF BLUNT SNOUT BREAM (MEGALOBRAMA AMBLYCEPHALA)

用LKB水平电泳仪,4.4%聚丙烯酰胺凝胶,对10—11月龄团头鲂(全长14—16cm)的11种组织——白肌、晶体、脑、脾、肾、性腺、心、肝、鳍、红血球及血清的乳酸脱氢酶和苹果酸脱氢酶作电泳,再用LKB激光光密度计对同工酶的迁移率及活性作定性定量分析。在不同组织中,同工酶的迁移率和活性有明显的组织特异性。

In LKB electrophoresis set with 4.4% polycrylamide gel, the lactate dehydrogenase(LDH) and malate dehydrogenase (MDH) were examined from 11 tissues-white muscle, eye lens,brain, liver, kidney, gonad, heart, spleen, fin, red cell and serum of blunt snout bream. Qualitativeand quantitative analysis on mobility and activity were conducted by a LKB Ultorscan laser de-nsitometer. The activity and mobility of isozymes in different tissues showed a significant tissuespecificity.