利用培养的同种异体皮肤成纤维细胞制作aPVR动物模型

Cultured homologous dermal fibroblasts of rabbit in aPVR animal model

目的 :利用培养的同种兔皮肤成纤维细胞制作前部增生性玻璃体视网膜病变 (aPVR)动物模型 ,为防治aPVR引起的慢性低眼压症提供实验基础。 方法 :利用培养的同种兔皮肤成纤维细胞制作aPVR动物模型 ,于术后不同时间点分别进行大体标本、组织病理观察。 结果 :大体标本显示 ,术后 2 8和 5 6天实验组周边视网膜牵拉性脱离。光镜检查发现 ,实验组 2 8天和 5 6天睫状体无色素上皮萎缩、缺失。 结论 :在aPVR病理状态下 ,睫状膜的增生和收缩引起对睫状上皮的牵拉 ,造成睫状体无色素上皮萎缩 ,可能是引起慢性低眼压症的主要原因。

Objectives:To establish animal model of anterior proliferative vitreoretinopathy (aPVR) with cultured homologous dermal fibroblasts of rabbit, and to provide evidence why hypotony was caused by aPVR. Methods:Animal models of aPVR were established with cultured homologous dermal fibroblasts on pigmented rabbits. Rabbits were sacrificed on the 14th, 28th and 56th day after the operation to prepare naked eyes and to receive histological examinations. Results:Naked eye examination showed that the peripheral retina was detached by dragging in the experimental group 28 and 56 days postoperatively. Microscopic examination showed atrophy or absence of the non-pigmented ciliary epithelium on the 28th and 56th postoperative day in the experimental group. Conclusions:The epiciliary membrane in aPVR dragged the ciliary body, made atrophy of non-pigmented epithelium, which perhaps was the main cause of hypotony.