摘要
本文对绒毛白蜡微体快速繁殖技术进行了研究,试验采用单芽茎段作为外植体,结果表明:BA、GA、活性炭、碳源对嫩梢增殖生长有影响,MS+BA_(3.0-4.0)+IBA_(0.1)+蔗糖3—4.5%最利于嫩梢的形成。间苯三酚、IBA浓度和光照影响根的诱导,MS+IBA_(1.5)+PG_(60-120)+蔗糖3%最利于生根,黑暗条件下能提高生根率,提早生根时间。本文还提出了绒毛白蜡微体快速繁殖的优化技术程序。
The techniques for rapid micropropagation of velvet ash are described. The stem sections with a bud were used as explants. The results showed: BA, GA, AC, active carbon and carbon source had effects on shoot growth. MS+BA_(3.0-4.0)+IBA_(0.1)+sucrose 3—4.5% was the best medium for shoot regeneration. The rooting of shoots was affected by IBA concentration, PG and light. MS+IBA_(1.5)+PG_(60-120)+sucrose 3% was the best medium for rooting.
出处
《山东农业大学学报（自然科学版）》
CSCD
1989年第3期61-65,共5页
Journal of Shandong Agricultural University：Natural Science Edition
关键词
绒毛
白蜡
繁殖
定芽增殖
micropropagation
bud multiplication
velvet ash
