生脉注射液对重症急性胰腺炎早期多器官组织脂质过氧化的影响

Effect of SHengmai Injection on Lipid Peroxidation of Multiple Organs in Rats with Severe Acute Pancreatitis of Early Stage

目的 :探讨生脉注射液对重症急性胰腺炎早期多器官组织脂质过氧化的影响及其作用机制。方法 :采用逆行胰管注射法建立大鼠重症急性胰腺炎模型 ,观察胰、心、肝、肺、肾组织的超氧化物歧化酶 (SOD)、丙二醛 (MDA)和组织学变化及生脉注射液的影响。结果 :模型组大鼠多器官组织中MDA含量较正常组明显升高 ,SOD活性较正常组明显降低。生脉组大鼠多器官组织中MDA含量显著低于模型组 ,SOD活性显著高于模型组。结论 :生脉注射液可显著减轻重症急性胰腺炎早期多器官组织脂质过氧化 ,提高多器官组织SOD活性是其作用机制之一。

Objective:To investigate the mechanism of Shengmai Injection in the early stage treatment of severe acute pancreatitis.Methods:The levels of lipid peroxide(MDA)、superoxide dismutase(SOD) and the histologic changes of pancreas、lung、kidney、liver、heart were measured after the rats with SAP were induced by retrograde injection of 5% sodium taurocholate into hepatopancreatic duct.The changes of those indexes were also measured after Shengmai Injection were injected intramuscularly just after induction of SAP.Results:The levels of MDA in Multiple Organs in SAP group were significantly higher than those in sham operation group;while the levels of SOD in relevant tissue in SAP group were significantly lower than those in sham operation group.The levels of MDA in Multiple Organs in Shengmai Injection group were significantly lower than those in SAP group;while the levels of SOD were significantly higher in relevant tissue.Conclusion:Shengmai injection may attenuate the level of lipid peroxidation in multiple organs of SAP,and their effect of increasing SOD level in relevant tissue may account for it.