摘要
以ESN/AES-IGSS法与ELISA法相对照,检测了135份血清的抗双链DNA抗体。结果表明,ESN-IGSS、AES-肝-IGSS、AES-肾-IGSS与ELISA的符合率分别是91.1%、95.6%和95.6%。对SLE的敏感性ESN-IGSS为82%,在统计学意义上高于ELISA的68.9%(P<0.05);AES-肝/肾-IGSS均为73.8%,略高于ELISA,但无统计学意义(P>0.05)。对SLE的特异性,ESN-IGSS、AES-肝/肾-IGSS三法分别为91.8%、93.2%和93.2%,与ELISA的91.8%比较,差异无统计学意义(P>0.05)。因而进一步证明ESN/AES-IGSS法之可靠。
Anti-dsDNA antibodies ( AdsDNA ) in 135 sera were tested by iramu -nogold silver staining (IGSS) with enzymes-treated human spermatozoal nuclei ( ESN ) or HCI-treated mouse liTer/kidney imprints ( AES-L/AES-K ) used as the substrate and simultaneously by,enzyme-linked immunosorbe-nt assay ( RLISA ) to compare the results obtained . The accordant rates of the results obtained from IGSS using ESN, AES-L and AES-K, with that obtained from ELISA were 91.1%, 95.6% and 95.6% respectively.To SLE , the sensitivity of IGSS was 82% when ESN was the substrate , significantly higher than that of ELISA (68.9%) ( P<0.05 ); but was 73.8% when AES-L or AES-K was nsed, slightly but not significantly higher than that of ELISA (P<0.05).The specificities of ESN-IGSS, AES-L-IGSS and AES-K-IGSS were 91.8%, 93.2% and 93.2% respectively, while that of ELISA was 91.8%, the differences not significant ( P>0.05). These results further show that the methods of ESN/AES-Igss for AdsDNA are reliable.
出处
《徐州医学院学报》
CAS
1993年第2期83-86,共4页
Acta Academiae Medicinae Xuzhou
关键词
DNA
抗体
ELISA
免疫金银染色
immunoassay
anti-dsDNA antibody ( AdsDNA )
enzyme-linked immunosorbent assay ( ELISA )
immunogold silver staining ( IGSS )