输血后红细胞及HLA抗体检测分析

ANALYSIS OF HLA AND RED BLOOD CELL ANTIBODIES AFTER TRANSFUSION

观察了304例患者输血后红细胞及HLA抗体产生情况。304例中7人检出红细胞抗体,占2.3％,均为抗-E,输血次数多者易产生抗体。7例抗-E阳性的病例中2例并发溶血性反应。各种疾病之间红细胞抗体阳性率无差异。119例血清内HLA抗体阳性,占39.14％,非溶血性输血反应与HLA抗体有密切关系。

A study was performed to estimate the frequency of alloimmunization against red cell ( RBC ) antigens in 304 multiply-transfused patients . The sera were investigated 3 , 7 and 14 days after the latest transfusion . It was found that 7 patients (2.3%) produced RBC antibodies, all of which were anti-E. The incidence of antibodies formation was increasing with the number of transfusions. In the 7 anti-E positive cases, 2 were complicated with hemolytic transfusion reactions ( HTR ) , while the other 5 were not. The possible pathogenesis of HTR was hypothesized. The proportion of patients with different diseases did not vary significantly in producing RBC antibodies. Of the 304 patients, 119(39.14%) produced HLA antibodies. A close relationship between nonhemolytic transfusion reactions ( NHTR ) and the development of HLA antibodies was demonstrated.