ESN/AES-IGSS法与RIA法检测抗双链DNA抗体的比较

COMPARISON BETWEEN ESN/AES-IGSS AND RIA FOR ANTIBODIES TO DSDNA

通过对118份血清抗双链DNA抗体的检测,比较了酶处理人精子头核/酸洗脱鼠肝肾印片免疫金银染色法(ESN/AES-IGSS)与放射免疫测定法(RIA)的检测结果。IGSS试验与RIA的符合率ESN为93.2％,AES-肝/肾各均为90.7％;对SLE的敏感性、特异性均与RIA相当(P均>0.05);且此3种底物上IGSS阳性滴度均与RIA dsDNA结合率呈密切的直线正相关。故ESN/AES-IGSS法的可靠性被进一步证实。但如血清稀释在ESN-IGSS<1:20、在AES-IGSS<1:10时,ESN、AES-肝/肾3底物上IGSS检测结果与RIA的符合率分别降为88.14％、86.44％和85.59％,反映了底物中微量非特异因子对高浓度血清检测结果的干扰。

By testing 118 sera for antibodies to dsDNA, the enzymes-treated human spermatozoa nuclei ( ESN )/acid-elution-treatdd substrates ( AES ) of mouse liver and kidney imprints immunogold-silver staining ( IGSS ) was compared with the traditional classical method of radioimmunoassay ( RIA ) . It was found that the coincident rates of the results from RIA and from IGSS with the substrates of ESN, AES-L and AES-K were 93.2%, 90.7% and 90.7% respectively, the sensitivities and specificities of IGSS with ESN, AES-L and AES-K were all similar to tl-ose of RIA (P>0.05) , and there were close positive linear correlations between the titers in positive IGSS and the radioactive dsDNA binding rate in RIA. The reliability of ESN/AES-IGSS was confirmed again thereby. As sera diluted less than 1 : 20 in ESN-IGSS, or less than 1:10 in AES-IGSS, how ver, the coincident rates dropped to 88.14%, 86.44% and 85.59% respectively. This reflected the'interferences of non-specific microelements in the substrates with the detection at lower serum dilutions.