水貂犬瘟热、病毒性肠炎和肉毒梭菌中毒三联疫苗的研究

STUDIES ON MINK DISTEMPER, VIRUS ENTERITIS AND BOTULISM COMBINATION VACCINE

用FPV弱毒、CDV弱毒和肉毒梭菌C型疫苗研制成功了水貂三联疫苗。该苗安全可靠、免疫原性强、免疫期达6个月以上,同各单苗相比抗体产生期及效价相近,室温可保存3天;4℃可保存2周;-20℃可保存6个月。该苗的免疫剂量为2ml,其中CDV和FPV弱毒滴度分别为10^(3.0),肉菌梭菌C型疫苗为2mg/ml。现场应用26000余只水貂,抗体阳转率达90％以上。

Modified FPV, CDV and botulism Type C vaccines were used to prepare mink three combination vaccine. Vaccination experiments show that the vaccine is safety, its immu-nologenicity is high, the immune period is up to six months. Compared with mink distemper modified virus vaccine, mink enteritis modified virus vaccine and botulism vaccine respectively, the combination vaccine has a similar antibody production period and titers. The vaccine can be stored at room temperature for 3 days; at 4℃ for 2 weeks; at - 20℃ for six months. The immune dosage of the vaccine is 2ml. The titers of modified CDV and FPV are 103.0; that of botulism Type C vaccine is 2mg/ml. The vaccine was used in above 26000 minks in the field. The antibody converting ratio is up to 90 percent.