肠艾美耳球虫内生发育阶段超微结构研究——Ⅲ.小配子发育

ULTBASTBUCTUBAL STUTY OF ENDOGENOUS STAGES OF EIMEBIA INTESTINALIS—— Ⅲ. MICROGAMETOGENESIS

肠艾美耳球虫(Eimeria intestinalis)小配子体及小配子的发育均在宿主细胞的带虫空泡内进行,带虫空泡内含有较多的泡内小管以及由小配子体逸出的线粒体和基质小体。多核小配子体是由最初的单核小配子体发育而成。小配子的形成首先是中心粒转变成鞭毛的基粒,此后鞭毛和顶体与小配子体成切线方式长入带虫空泡;几个小线粒体融合成一大线粒体,嵌于核前方一侧,与核一起突入带虫空泡。成熟的小配子体长约6.5μm,有两根鞭毛。鞭毛的横断面为9+2的微管结构。体部由顶体、线粒体和核组成。由顶体的腹侧向后发出4～6根微管,其中只有2～3根延伸到体后部。

The development of microgametocytes and microgametes of E. intestinalis lay within the parasitophorous vacuole. There were some intravacuolar tubules, mitochondria and cytoplasma bodies within the parasitophorous vacuole. Microgamete formation occured when two centrioles developed into based bodies of the flagella, then the flagella and the perforatorium grew up into the parasitophorous vacuole. Small mitochrodria fused into the large mitochrodrion. The mitochrodrion was in close association with the nucleus and protruded into the parasitophorous vacuole. The mature microgamete had an elangate crescent-shaped body of about 6.5μm long and two flagella. Each flagellum had nine peripheral fibers and two central fibers in cross sections of the microgamete. The perforatorum was the anterior pole of microgamete. A large mitochrodrion was situated in front of the elongate nucleus. Four to six microtubules were originating in the ventral side of the perforato-rium, and two or three of them extend posteriorly to the posterior extremity.