产蛋鸡与休产鸡肝脏、输卵管雌激素受体含量的比较

QUANTITATIVE STUDY ON ESTROGEN RECEPTORS OF HEN LIVERS AND OVIDUCTS

本试验选用正在产蛋和休产的来航鸡,用放射配体结合分析法,分别测定两组鸡的肝脏和输卵管胞浆和胞核受体含量。胞浆液的蛋白含量用考马斯亮蓝G250法测定;胞核DNA含量用紫外吸收法测定。试验结果表明:两组鸡的肝脏和输卵管的胞浆雌激素受体含量均无明显差异;两组鸡的肝脏和输卵管的胞核雌激素受体含量,产蛋鸡肝脏的胞核雌激素受体是休产鸡的15倍,差异显著(P<0.05),而输卵管的胞核雌激素受体差异不明显。

Using radio-ligand binding assay, we quantified the estrogen receptors in the liver and oviduct of laying and non-laying hens. The cytoplasmic estrogen receptor contents in the liver and oviduct of the two groups of hens were very low. There was no significant difference between the two groups. The concentrations of estrogen receptors in the nuclear fractions of liver tissue between the two groups were significantly different: 3.72 picomoles/mg DNA in laying, and 0.25 picomoles/mg DNA in non-laying. No significant difference in nuclear estrogen receptor contents of oviduct was found between the two groups of hens.