发酵液中L-精氨酸定量检测方法的研究

Study on the method of L-arginine quantification in fermentation broth

确立了用甲萘酚-双乙酰法定量检测发酵液中L-精氨酸 的最佳条件:40g/LNaOH溶液、80g/L甲萘酚正丙醇溶 液、0.5mL／L双乙酰正丙醇溶液各1mL作为显色液,再加 入1μL适当稀释的发酵液,30℃水浴加热15min后测其 OD540值。对显色反应的稳定性及检测方法的重现性也进 行了研究。

In this paper, the optimal conditions for quantitative determination of L -arginine in fermentation broth were established: 1 ml of 40g/L NaOH, 80g/L 1 -Naphthol -propanol and 0.5mL/L Diacetyl -propanol was added sequentially as the indicator, then 1μL of appropriately diluted broth was added. The mixture was incubated in a water bath at 30℃ for 15 minutes, and then its OD value was measured at 540nm. The stability of this reaction and the reproducibility of this method were also studied.