摘要
确立了用甲萘酚-双乙酰法定量检测发酵液中L-精氨酸 的最佳条件:40g/LNaOH溶液、80g/L甲萘酚正丙醇溶 液、0.5mL/L双乙酰正丙醇溶液各1mL作为显色液,再加 入1μL适当稀释的发酵液,30℃水浴加热15min后测其 OD540值。对显色反应的稳定性及检测方法的重现性也进 行了研究。
In this paper, the optimal conditions for quantitative determination of L -arginine in fermentation broth were established: 1 ml of 40g/L NaOH, 80g/L 1 -Naphthol -propanol and 0.5mL/L Diacetyl -propanol was added sequentially as the indicator, then 1μL of appropriately diluted broth was added. The mixture was incubated in a water bath at 30℃ for 15 minutes, and then its OD value was measured at 540nm. The stability of this reaction and the reproducibility of this method were also studied.
出处
《食品工业科技》
CAS
CSCD
北大核心
2005年第2期184-186,共3页
Science and Technology of Food Industry
关键词
L-精氨酸
发酵液
定量检测
L-arginine
broth
quantitative-determination