2709碱性蛋白酶酶解鱼鳞工艺及其产物的功能特性

The Hydrolysis of Fish Scale and Its Properties

应用正交实验优选鱼鳞蛋白酶解的最佳条件。采用 2 70 9碱性蛋白酶 ,考察了酶量、固形物浓度、反应温度和酶解时间等因素对酶解反应的影响 ,确定最适条件。pH的选择参照了已有实验酶的最适 pH值 ,以水解度、游离氨基酸量为检测指标。结果表明 ,鱼鳞酶解宜采用碱性蛋白酶 2 70 9,用盐酸和氧化钙作软化的预处理 ,可使鱼鳞蛋白得到较高程度的水解 ,酶解最佳温度为 5 0℃ ,酶量宜采用 3 0 % ,酶解的最佳时间为 5h ,底物浓度宜选择 10 %。鱼鳞水解蛋白有很好的吸水性、乳化性、起泡性和溶解性 ;水解蛋白对羟基自由基也有很好的清除能力 ,其IC50 为 2 1mg/mL ,最大清除率为 93 6 %。

The hydrolysis conditions of fish scale using 2709 alkaline protease and founctional properties of the product were studied. The optimum conditions were found by mono-factor analysis and orthogonal test after fish scale was pre-treated with HCl and CaO, and the conditions are 10% concentration of the substrate, pH 8～9, 50℃ hydrolyzing temperature, 3% ratio of enzyme and substrate.The hydrolysate possesses good solubility, emulsifiability, and foaminess. The hydrolysate also showed good scavenging to·OH, with maximun scavenging rate reached 93.6%.