金线莲组织培养中若干因素的研究

Studies on Some Influencing Factors in Tissue Culture of Anoectochilus roxburghii

研究了影响金钱莲组培芽的增殖和营养器官分化的几个因素。结果表明:植物细胞分裂素及生长素的种类、浓度和配比对瓶苗茎发育有很大影响,在增殖期使用浓度为1.0mg/L BA、0.5mg/L IBA,器官分化生长期使用浓度为增殖期激素量的三分之一;瓶移植量10～15株;光照量显著影响植株生长期叶片生长,500lx较合适;活性炭明显提高诱导根的生长质量,添加浓度0.5％效果最好。

Some factors influencing shoot proliferation and differentiation of vegetative organs in tissue culture of Anoectochilus roxburghii were studied. The results showed that different kinds concentrations and proportioning of cytokinin and auxin had great effcts on the development of in vitro seedling stems. During proliferation, the concentration was : BA 1. 0 mg/L, IBA 0. 5 mg/L, while at differentiation and development of organs, the concentration of hormones reduced 3 times than that at proliferation stage; 10-15 plantlets were transplanted.The intensity of illumination markedly influenced the leaf growth at the plant growth stage. 500 lx was optimum intensity. Active carbon significantly improved the growth of induced roots. Better result was obtained when added with 0. 5% active carbon.