诺氟沙星的荧光光谱研究及其应用

The Fluorescence Characteristics of Norfloxacin and Its Application

采用荧光光谱法研究了诺氟沙星在不同 pH条件下的光谱行为 ,发现诺氟沙星在pH 3 0 4的水溶液中具有较强的荧光发射 ,与中性介质相比荧光发射光谱红移了 30nm。据此分别建立了药物制剂中和人体尿液中诺氟沙星的荧光光谱法和同步 导数荧光光谱法。经样品测定 ,其线性范围为 0 0 16～ 0 96 μg·mL-1,检出限为 0 0 16 μg·mL-1,回收率为 99 7%～ 10 0 94 % ,相对标准偏差为 1 6 5 %～ 2 86 %。

The fluorescence characteristics of norfloxacin in different pH media have been studied by spectrofluorimetry. It was found that in the neutral and alkalescent media the fluorescence signal of norfloxacin was weak, while in the acidity media the signal was very strong. The maximum fluorescence signal of norfloxacin could be obtained at pH 3.04, and the norfloxacin fluorescence emission wavelength has red-shifted 30 nm compared with the other condition. Based on this, a spectrofluorimetric method of the determination of norfoxacin in pharmaceuticals is proposed. The linear range was 0.016-0.96 mug(.)mL(-1), the detection limit was 0.016 mug(.)mL(-1), the RSD was 1.65%-2.86%, and the recovery was within the range of 99.7%-100.94%. At pH 3.04, norfloxacin in human urine can be well resolved by Synchronization-1st derivative fluorescence spectroscopic techniques with Deltalambda = 90 nm, and the interference from urine blank can also be eliminated. The linear range was 0.096-3.19 mug(.)mL(-1) for determining norfloxacin in human urine sample. The detection limit was 0.096 mug(.)mL(-1), the recovery was within the range of 96.24%-104.4% and RSD was 0.71 %-2.60%. Pharmacokinetic study of norfloxacin showed that norfloxacin reached its peak concentration in urine samples two hours after a healthy man took capsules. The method is simple, rapid and accurate with high sensitivity and good selectivity, and suitable for the determination of norfloxacin. The method was applied to analysis of norfloxacin in drugs and biological samples.