胃癌组织内树突状细胞的浸润及其预后意义

Dendritic cells infiltration in gastric cancer tissues for its prognosis

目的 :研究胃癌组织内树突状细胞(dendriticcell ,DC)的浸润及其对预后的影响。方法 :将S 10 0蛋白抗体作为DC特异性标记物 ,对 3 0例胃腺癌组织进行免疫组化染色和DC定量计数。结果 :高分化和低分化胃癌组织中每个高倍镜视野的阳性细胞数分别为2 1 43± 12 0 5和 10 45± 13 5 4,两组DC数量比较差异有统计学意义 ,P <0 0 5 ;有淋巴结转移组和无淋巴结转移组平均每个视野中的阳性细胞数分别为 6 90± 4 43和 15 99± 8 3 6,无淋巴结转移的DC数量明显高于淋巴结转移者 ,P <0 0 0 1;术后生存期 <5年和≥ 5年的两组DC计数分别为4 68± 3 2 9和 10 47± 7 71,P <0 0 5。结论 :胃癌组织中DC的浸润明显延长胃癌患者的生存 ,且与淋巴结转移有关 。

OBJECTIVE: To study the dendritic cell (DC) infiltration in gastric cancer and its relationship with the tumor different grade, lymphatic metastasis and the survival rate.METHODS:Thirty patients with gastric cancer were grouped according to the different grade, metastasis and 5-year survival status. The DC infiltration were histopathologically identified by the immunohistochemical stain using S-100, a special antibody to DC.RESULTS:The numbers of the DC infitration were counted to be significantly more in the high-differentiation tumors than those in the low-differentiation tumors, P<0.05; and more in the group without lymphatic metastasis than in the group with lymphatic metastasis, P<0.001; and more in ≥5-year survival group than that in <5-year survivals, P<0.05. CONSLUSIONS:The dentritic cell infiltration in gastric cancer has relation to the tumor different grade and lymphatic metastasis. It can be used as an indicator of prognosis of the patients.