卵巢癌端粒酶逆转录酶和脆性三联组氨酸蛋白表达的临床病理分析

Expressions of telomerase reverse transcriptase and fragile histidine triad in human ovarian carcinoma

目的 :探讨卵巢癌组织中端粒酶、脆性三联组氨酸蛋白表达状况和临床病理的关系。方法 :用端粒酶逆转录酶 (telom erasereversetranscriptase ,TRT)、脆性三联组氨酸 (fragilehistidinetriad ,FHIT)蛋白抗体和免疫组织化学方法检测 3 4例卵巢癌、14例囊腺瘤和 10例正常卵巢标本 ,并结合年龄、组织学分类、分级和临床分期进行分析。结果 :卵巢癌组织均表达TRT ,其中强阳性 79 4% ( 2 7/3 4) ,弱阳性 7例 ;14例囊腺瘤及 10例正常卵巢上皮TRT表达均为弱阳性。癌组织与囊腺瘤及正常上皮组织比较 ,差异有统计学意义 ,P <0 0 0 1。卵巢癌组织FHIT表达阳性 47 1% ( 16/3 4) ,阴性 18例 ;14例卵巢囊腺瘤组织FHIT表达阳性者为 85 7% ( 12 /14 ) ;10例卵巢正常形态组织FHIT表达均为阳性 ;组间比较差异有统计学意义 ,P <0 0 5、P <0 0 1。TRT表达与卵巢癌分期差异有统计学意义 ,P <0 0 5 ;FHIT表达与年龄、组织学分类、分级和分期无相关性 ,P >0 0 5。结论 :TRT表达升高、FHIT表达降低或缺失可能与卵巢癌的发生有关 ;

OBJECTIVE:To investigate the clinicopathological significance of the telomerase reverse transcriptase (TRT) and the fragile histidine triad (FHIT)expressions in human ovarian carcinoma. METHODS:The expressions of TRT and FHIT in 34 cases of formalin-fixed, paraffin-embedded ovarian carcinoma tissue were detected by using antibody to human TRT and FHIT with Streptavidin-HRP (SP) immunohistochemical method, and analysed with clinicopathological data.RESULTS: Expression of TRT was found positive in all 34 cases of ovarian carcinoma, of which 27 cases (79.4%)were strongly positive and 7 weakly positive. Fourteen cases of cystic adenoma and 10 normal ovarian epithelia were weakly positive for TRT. There was a significant difference in TRT expression between cancer and adenoma as well as normal ovary,P<0.001. Expression of FHIT protein was found in 47.1% (16/34) of ovarian carcinomas, 85.7% (12/14) of cystic adenomas and 10/10 of normal ovarian tissues. There was also a significant difference in FHIT expression between carcinomas and cystic adenomas,P<0.05,or normal ovarian superficial epithelia,P<0.01. Expression of TRT,but not FHIT was related to the clinical stage,P<0.05. Both TRT and FHIT expressions in carcinoma were not corresponding to age,and the histological type,or grade, P>0.05. CONCLUSIONS: The increased expression of TRT and the decreased expression of FHIT may be associated with the carcinogenesis of ovarian superficial epithelia. Both TRT and FHIT expressions may be considered as the new adjunct diagnostic and prognostic markers in ovarian tumor.