全氟辛磺酸对大鼠中枢神经系统谷氨酸含量的影响

Effects of perfluorooctanesulfonylsulfonate on glutamate concentration of central nervous system in rats

目的 探讨全氟辛磺酸 (PFOS)对大鼠中枢神经系统谷氨酸能神经元的影响 ,以探讨PFOS对神经系统的毒作用机制。方法 成年雄性Wister大鼠用PFOS经口 1次染毒 ,实验组剂量分别为 5 0、10 0和 2 0 0mg kg ,对照组给予等体积的体积分数为 2 %的吐温 80溶液。 2 4h后用体积分数为 10 %的中性缓冲甲醛灌流处理并利用免疫组织化学方法和显微图像分析技术 ,分析大鼠脑组织切片中谷氨酸反应阳性细胞的阳性面积比、平均积分吸光度。结果 PFOS染毒 2 4h后大鼠大脑皮层、海马、小脑中阳性面积比、平均积分吸光度与对照组相比 ,明显升高且有显著性 (P <0 0 1)。结论 中枢神经系统中谷氨酸含量的升高可能在PFOS引起大鼠神经毒性的机制中起重要作用。

Objective This study was conducted to explore the effects of perfluorooctanesulfonylsulfonate(PFSO) on glutamatergic neurons in rat central nervous system.Methods Groups of male Wistar rats were exposed once with 50,100,200 mg/kg PFOS orally.The controls were treated with 2% tween-80.The percentage of positive area and integral optical density of glutamate reactive cells in rats CNS were evaluated by immunohistochemical method at the other day after the rats were decapitated and perfused with 10% buffer formaldehyde solution through left ventricle.Results The percentage of positive area and integral optical density of glutamate immunoreactive cell increased significantly (P<0.01) in comparison with control in cerebral cortex,hippocampus and cerebellum.Conclusion It is suggested that glutamate is likely to play a role in the excitatory neurotoxicity of PFOS.