皮下注射雨蛙肽对大鼠应激性胃粘膜损伤的影响

Effect of Subcutaneous Injection of Caerulein on Stress Induced Gastric Mucosal Lesion in Rats

探讨皮下注射雨蛙肽对束缚-浸水引起的大鼠应激性胃粘膜损伤的影响及其机理。结果:①皮下注射雨蛙肽,可明显地减轻大鼠应激性胃粘膜损伤,并在一定的剂量范围内呈现量效关系;②预先皮下注射消炎痛(5mg/kg),可阻断雨蛙肽的抗胃粘膜损伤作用;③皮下注射雨蛙肽,可降低应激大鼠胃粘膜中丙二醛的含量;④电镜观察结果,皮下注射雨蛙肽能抑制应激大鼠胃粘膜壁细胞胃酸分泌的增加。结果表明,雨蛙肽的抗胃粘膜损伤作用可能由内源性前列腺素所中介,并与其清除自由基有关,还通过抑制应激大鼠的胃酸分泌而实现。

Observed the effect of subcutaneous injection of caerulein on stress induced gastric mucosal lesion in rats. The results obtained were as follows: 1)Subcutaneous injection of certain doses caerulein significantly reduced the stress induced gastric mucosal lesion in rats in a dose-dependent manner. 2)The effect of caerulein was abolished by indomethacin(5mg/kg). 3)Subcutaneous injection of caerulein significantly reduced the content of malondialdehyde in gastric mucosa in stressed rats. 4)Subcutaneous injection of caerulcin(20ng/kg) did not influence the secretion of the gastric juice in normal rats. 5)In electron micrographs, the subcutaneous injection of caerulein inhibited the increase of the secretion of gastric parietal cells. The results mentioned above indicate that the effect of caerulein is caused by promoting synthesis of PG, scavenging free radical and inhibiting secretion of gastric juice.