非接触镜使用者的棘阿米巴角膜炎一例

Acanthamoeba Keratitis in Non-Contact Lens Wearers: one case report

将一例环形角膜溃疡的手术切除角膜材料,用2%无营养无盐琼脂培养基加大肠杆菌肉汤进行培养,成功地分离出了棘阿米巴原虫,原虫在该培养基上生长良好,并可传代生长。培养物经10%氢氧化钾湿封片,显微镜下观察,可见棘阿米巴原虫的双层壁包囊。患者的典型表现为角膜环形基质浸润,伴剧烈眼痛。经角膜板层切除加结膜瓣掩盖手术及术后药物治疗,痊愈出院。

A corneal material from ring-shape corneal ulcer was cultured in small Petri plates containing Ioml of 2% nonnutrient, non-salt agar, and one drop of young E. coli broth was placed over the inaculum, We have successfully isolated an Acanthamoeba which could grow very well in this cultural medium and could grow by passage culture. Cultural material was examined by 10% potassium hydronide wet mount, doublewalled polyginal cycts of Acanthamoeba were clearly observed under ordinary light microscopy, Patient's characteristics were corneal ring-shaped stromal infitrate and extreme ocular pain. Patient was completely recovered by corneal lamelar kerectomy combined with keratoleptynsis and postoperation medical treatment.