摘要
本文对火棘水溶性多糖的提取工艺、分离纯化以及纯度鉴定作了研究。结果表明:火棘多糖的最佳提取工艺为90℃、9h、料液比为1:10,火棘多糖的提取率约为3.5%;通过分级沉淀、DE-52柱层析分离得到PP-A2、PP-A3、PP-A4和PP-B1、PP-B2、PP-B3六种组分;经SephadexG-200柱层析鉴定,PP-A2、PP-A3、PP-B2为均一多糖。
The extraction, isolation and purification of water-soluble Pyracantha fortuneana polysaccharides were studied. The results indicated that the optimal extraction technology of Pyracantha fortuneana polysaccharides were 90℃, 9h and 10:1(W/W) of water to its fruit. Extraction rate of the polysaccharides was 3.5%. Then through isolating it with fractional precipitation and DE-52 column chromatography, six glycoconjugates, PP-A2, PP-A3, PP-A4, PP-B1, PP-B2 and PP-B3, were obtained. Sephadex G-200 gel filtration chromatography showed that PP-A2, PP-A3, PP-B2 were pure.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第1期157-161,共5页
Food Science
关键词
火棘
多糖
提取
分离纯化
Pyracantha fortuneana
polysaccharides
extraction
isolation and purification
