摘要
本文采用酶法制备滑菇多糖(PholiotNamekoPolysaccharide,PNP),确定了制备的最佳工艺。加酶(纤维素酶和中性蛋白酶)酶解后,热水浸提,再用sevage法除去蛋白,95%的乙醇醇析得到滑菇多糖的纯化物。
This paper mainly investigated the preparation, purification and determination of Pholiot nameko polysaccharide (PNP). The optimum processing of the PNP was introduced. The process was as follows: Neutral proteinase and water were mixed with Pholiot nameko and kept in certain temperature and extracted by hot water. The protein in crude polysaccharide was removed by sevage method and purified with 95% ethanol.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第1期161-164,共4页
Food Science
关键词
滑菇多糖
工艺
测定方法
namko polysaccharide
process
method of determination