GITC柱前手性衍生化HPLC测定吡喹胺光学异构体纯度

DETERMINATION OF THE OPTICAL PURITY OF PRAZIQUANAMINE BY REVERSED PHASE HPLC AFTER DERIVATIZATION WITH GITC

以乙酰葡萄糖异硫氰酸酯(GITC)作柱前手性衍生化试剂,用反相高效液相色谱法成功地拆分了吡喹胺(1,3,4,6,7,11b-六氢-2H吡嗪[2,1-a]并异喹啉-4-酮)光学异构体。在此基础上建立了测定吡喹胺光学异构体纯度的方法,并已用于d-和l-吡喹胺纯度的测定。

The enantiomers of praziquanamine were separated by reversed phase HPLC after derivatization with 2,3,4,6- tetra- O- acetyl- β-D- glucopyranosylisothiocyanate (GITC). The determination of the optical purity of praziquanamine was successfully achieved. This method was applied to the determination of the optical purity of d-and l-praziquanamines.