裸鼠同系肿瘤细胞DNA对SF_8传代细胞的体外转染

IN VITRO TRANSFECTION OF SF8 CELL LINE BY ISOGENEIC TUMOR CELL DNA

SF_8是一弱转化成纤维细胞系,来源于同系移植的BALB/c裸鼠淋巴肉瘤的间质。本实验证明,SF_8传代细胞可被同系淋巴肉瘤细胞的DNA转染,发生进一步地恶性转化,变为强转化的成纤维细胞(SFtr)。

From our previous studies,we found that the isogeneic transplanted lymphoma cells could induce partial transformation of stro- mal fibroblasts of BALB/c nude mice,known as Horizontal Transforma- tion.In this report,we further demonstrate that the partially transformed cell line(SF_8)can be transfected by isogeneic lymphoma cell DNA in vitro,resulted in fully transformed cells.The possibility of multistep nature of Horizontal Transformation is discussed.