肾综合征出血热病毒负染法电镜观察

MORPHOLOGICAL STUDY ON HEMORRHAGIC FEVER WITH RENAL SYNDROME VIRUSES BY ELECTRON MICROSCOPY WITH NEGATIVE STAIN

在Ly-A6和Vero-E6细胞上接种7株肾综合征出血热病毒(HFRSV),用直接负染法、免疫负染法和免疫胶体金标记负染法制备了电镜标本,进行观察。7株病毒的形态与布尼亚病毒相似,但大小差别较大,为53～165nm,平均直径89.2nm,病毒包膜为脂质双层样,膜表面可见中空、突起的形态亚单位结构。

Seven strains of HFRSV from the supernatants of infected Ly-A6 and Vero-E6 cell lines were studied by electron microscopy with negative stain,immuno-negative stain,immunocolloidal gold labelled negative stain.Morphology of these HFRSV is similar to that of the viruses in the Bunyaviridae family.The average size of these viruses is smaller than other reported results.Morphological subunits,these are hollow projections,6-8nm in length and 5-7nm in diameter,can be seen on the surface of the viruses.It is suggested that these morphological subunits might represent a morphological feature of HFRSV.