摘要
采用乙腈为蛋白沉淀剂对血浆进行预处理,用均匀设计法安排实验,考察了血浆中硝普钠的代谢物硫氰酸盐与硝酸铁试液的反应条件。确定在456 nm测定其吸收度,测得SCN^-在1.68～13.4μg/ml的范围内与吸收度成线性关系(r=0.9999)。人体血浆中硫氰酸盐的相对平均回收率为94.0%,日内相对标准偏差在3.2%以下(n=10),日间相对标准偏差在4.0%以下(n=8)。最低检测限为0.3μg/ml。利多卡因等药物对测定无影响。
A sensitive, reproducible and rapid method for the spectrophotometric determination of thiocyanate, the metabolite of sodium nitroprusside, was developed. Acetonitrile was used to remove protein in the serum. Uniform design technique was used in arranging the experiments and choosing the reaction conditions between thiocyanate and ferric nitrate reagent. The wavelength of maximum absorption was 456 nm, the calibration curve of SCN^- was linear in the range of 1.68～13.4 μg/ml (r=0.9999), the relative average recovery of thiocyanate was 94.0%. The day-to-day and within-day relative standard deviations were less than 4.0% (n=8) and 3.2%(n=10) respectively. The limit of determination was 0.3 μg/ml. Drugs such as lidocaine, etc. presented no interference on the determination.
出处
《药学学报》
CAS
CSCD
北大核心
1993年第11期854-858,共5页
Acta Pharmaceutica Sinica
关键词
硝普钠
硫氰酸盐
分光光度法
Sodium nitroprusside
Thiocyanate
Spectrophotometry
Uniform design technique
