摘要
用聚合酶链反应技术对结核杆菌特异性重复序列ＩＳ６１１０部分基因１２３ｂＰ的片段进行扩增。该法检测人型结核杆菌灵敏度可达到１０ｆｇＤＮＡ，对１４９份结核患者临床标本检测结果显示ＰＣＲ总阳性率（６９．１％）明显高于直接涂片荧光镜检（１５．４％）与细菌培养（１０．１％）的阳性率（Ｐ＜０．０１）。研究表明ＰＣＲ是一种特异、敏感、快速诊断结核病的好方法，值得推广。
A 123bp segment derived from the repetitve DNA IS6110 specific for Mycobacteria tuberculosis was aplified by using poly merase chain reaction (PCR).The assay could detect as less as 10fg DNA of M.tuberculosis.It was used to patients with tuberculosis (67 sputa, 53 pleural fluid aspipositivity rate of PCR (69.1%) was signmificantly higher than that of conventional fluorescent staining(15.4%)and culture technique(10.1%)，Our results suggest that PCR may be a specific,sensitive and rapid technique for diagnosis of tuberculosis.
