枸杞多糖对运动性骨骼肌疲劳恢复的细胞定量化学分析和电镜观察

EFFECT OF LYCIUM BARBARUM POLYSACCHARIDE ON THE RECOVERY FROM KINETIC SKELETALMUSCLE FATIGUE──A STUDY WITH CELLU-LAR QUANTITATIVE CHEMICAL ANALYSIS AND ELECTRON MICROSCOPIC OBSERVATION

通过小鼠实验，用枸杞多糖解除运动性骨骼肌疲劳，以显微分光光度计进行琥珀酸脱氢酶（ＳＤＨ）和糖原的定性定量分析，并于透射电镜下观察超微结构的变化。结果显示，运动疲劳（Ｂ）组糖原含量明显降低，各类肌纤维与对照（Ａ）组比较Ｐ＜０．００１；ＳＤＨ活性增加，各类肌纤维与Ａ组比较Ｐ＜０．００１。服用枸杞多糖（Ｃ）组糖原含量增加，各类肌纤维与Ａ组比较Ｐ＜０．００１；与Ｂ组比较Ｐ＜０．００１；ＳＤＨ反应较Ｂ组更深，各类肌纤维与Ａ组比较Ｐ＜０．００１，若与Ｂ组比较，则其中Ｉ（慢肌）型和Ⅱ（中间肌）型Ｐ＜０．００１，而Ⅱ（快肌）型则Ｐ＞０．０５。超微结构显示，Ｂ组线粒体增多且体积明显增大，呈肿胀状态；Ｃ组全无此类改变，提示服用枸杞多糖后可解除疲劳，其中以Ｉ（慢肌）型肌纤维效果尤佳。

n experimental study on the elimination of kinetic skeletal muscuefatigue with administration of Lycium barbarum polysaccharide LBP wasmade.Qualitative and quantitative analyses of succinate dehydrogenase(SDH)and glycogen were made with microspectrophotometer,otherwiseultrastructural changes were also observed with transmission electronmicroscope.The results were as follows:Marked reduction of glycogen content in all sorts of muscle fibers took place in kinetic fatigue group(Group B)as compared with that ofthe control group(Group A)(P<0.001),and also with increase ofSDH activity(P<0.001),In the LBP.oral administration group(GroupC)the increase of glycogen content took place in all sorts of musclefibers in comparison with that of Group A and B (P<0.001,in both),and stronger reaction of SDH as compared with that of GroupB; Com-parison of all the muscle fibers with those of Group A, P<0.001; withGroup B: type I (slow muscle) and type Ⅱ (intermediate muscle) wereall P<0.01,but with type Ⅱ(fast muscle ),P>0.05.Ultrastructuralstudies revealed the increase in number and in bulk of mitochondria inGroup B, but no change in Group C,suggesting that LBP oral adminis-tration could eliminate fatigue,of which,the typeI(slow muscle) fiberswere shown the better effects.