摘要
目的 评价细胞周期蛋白E (cyclinE)非时相表达在胃癌发生发展中的意义。方法 收集新鲜胃癌组织标本 18例、正常胃粘膜组织标本 8例 ,采用机械法制备成单细胞悬液 ,应用流式细胞术 (FCM )检测胃癌组织和正常胃粘膜组织中cyclinE的表达及表达时相。结果 发现 33 8%的胃癌组织中有cyclinE的非时相表达 ,而相应的正常胃粘膜组织中无 1例出现非时相表达。结论 胃癌细胞中cyclinE的非时相性表达可能是引起细胞增殖紊乱 ,参与胃癌发生发展的重要分子事件之一。
Objective To investigate the unscheduled expression of cyclin E in gastric carcinoma. Methods The expression of cyclin E was detected in 18 samples of gastric carcinoma tissues and 8 samples of normal gastric tissues by multiparameter flow cytometry. Results The unscheduled expression of cyclin E was detected in 7 of 18 (33 8 %) gastric carcinoma tissues. And no unscheduled expression of cyclin E was detectable in normal gastric tissues. Conclusion Unscheduled expression of cyclin E in gastric carcinoma cells may cause growth pertubation of cell cycle and was one of important molecular events in development of tumor.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2003年第4期396-398,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
