胎儿输卵管上皮细胞分化观察

DIFFERENTIATION OF EPITHELIAL CELL IN THE HUMAN FATAL OVIDUCT

采用光镜和电镜技术观察了10周～38周胎儿输卵管上皮细胞特别是纤毛细胞的发育和分化。光镜下10周胎儿输卵管壶腹部管腔狭小,上皮为较原始的单层柱状上皮,12周粘膜上皮出现小的皱褶,16周～17周形成短的绒毛样突起,19周～32周渐形成较复杂的粘膜皱襞,至38周粘膜皱襞已接近成体。电镜下17周上皮中偶见单纤毛的细胞,胞质内细胞器较发达,糖原含量丰富,19周出现少量纤毛细胞,胞质细胞内器发达,可见溶酶体,此时纤毛较短,不具9×2+2微管结构,非纤毛细胞也有各种细胞器,但不含溶酶体。20周～30周纤毛细胞仍稀少,至32周以后纤毛细胞数量逐渐增多,其胞质中细胞器发达,糖原丰富,纤毛具典型的9×2+2微管结构,36周～38周可见形态分化较完好的纤毛细胞,非纤毛细胞顶端明显突向管腔,甚至落入管腔中,胞质内未见膜包的分泌颗粒。

Development of ciliated cells in the human fallopian tube was studied by light and electron microscope in speciments aged 10weeks～38 weeks.Under light microscope,the ampullary protion of the tube revealed a narrow lumen at 10 weeks.At 12 weeks,the lumen showed slight folding.At 38 weeks,the villous folding was similar to that of an adult.Under electron microscope,a solitary cilium was occasionally seen at 17 weeks,the epithelial cells rich in glycogenosame.At 19 weeks,the ciliated cells appeared,but cilia were shorter.After 32 weeks, ciliated cells with well-developed cytoplasmic organelles gradually increased,ciliated cells was rich in glycogenosame and cilia had a 9×2+2 microtubular structure.At 36weeks～38 weeks, numerous ciliated cells were observed,non-ciliated cells tended to protrude in the lumen,but secretory granules were not detected.