杂交瘤细胞培养保护剂的研究

Studies of Protective Agents for Hybridoma Cell Culture

利用鼠鼠杂交瘤２Ｆ＿７细胞（分泌ＩｇＧ＿２单抗），研究了普兰卢内克（Ｉ）、聚乙二醇（ＩＩ）、甲基纤维素（ＩＩＩ）、葡聚糖（ＩＶ）、聚醚多元醇（Ｖ）与杂交瘤细胞的生物相容性、添加限制浓度以及高速搅拌下添加剂对杂交瘤细胞的保护作用。实验结果表明，０．０５％～０．１％的Ｉ、０．０４％～０．２０％ＩＩ、０．１０％～０．２０％的ＩＩＩ能较好地保护杂交瘤细胞；ＩＶ会抑制细胞生长；ｖ会分解细胞。在１．５Ｌ生物反应器中培养，添加０．１０％Ｉ于培养基，搅拌转速为７０ｒ／ｍｉｎ时，细胞能正常生长。

The effects of different protective agents such as pluronic F-68,polyethyleneglycOl (PEG),methylcellulose(MC),dextran, on hybridoma 2 F_7 cell culture which secretesmonoolonal antibody against small cell lung cancer were studied. The potential toxicity,restricting adding concentration, protective effeccs at high agitation speed were investigated. It is shown that 0.05%～0.1%pluronic F-68,0.04%～0.2%PEG,0.1～0.2%MC canprotect hybrdoma cell from shear stress at high agitation-speed,dextran inhibits cell growth. In 1.5 L celligen bioreactor, adding 0.1% pluronic F-68 in medium,at 70 r/min of agitatingspeed,the hybridoma cells can normally grow.