摘要
目的:观察补阳还五汤对大鼠脊髓损伤后AQP 4表达和后肢恢复功能的影响。方法:100只SD大鼠 分为损伤组和中药组,均手术致脊髓不完全性损伤。中药组术后即给予补阳还五汤口服液灌胃;损伤组给予等量 蒸馏水灌胃。术后1、3、7、14及21d分别对大鼠进行BBB评分和斜板实验检查后肢功能,然后处死,应用免疫组 织化学技术检测脊髓组织AQP 4的表达。结果:脊髓损伤后第1天,2组受损伤脊髓灰质、白质中可见到AQP 4 的表达明显增加;第3天时达到高峰,损伤组更明显(P<0.05);第7、14及21天,中药组AQP 4表达的减少几乎 与神经功能的改善平行,与损伤组比较差异有非常显著性(P<0.01)。结论:补阳还五汤可能通过抑制脊髓损伤后 AQP 4表达,消除脊髓水肿,减轻脊髓继发性损伤,从而使残存脊髓组织保存并促进脊髓功能恢复。
Objective: To investigate the effect of Buyanghuanwu Tang formula on AQP-4 expression following spinal cord injury as well as functional recovery of rear limbs in the rats. Methods: 100 cases of SD rats were divided into injury group and Chinese traditional medicine group subject to operation on incomplete spinal cord injury(SCI). The Chinese traditional medicine group received Buyanghuanwu Tang formula after operation and injury group subject to identified quantity of distilled water.BBB scales and inclined plane tests were performed to test the function of the rear limbs, then the animals were sacrificed 1, 3, 7, 14 and 21 days after surgery. By using immunohistochemical method, the expression of AQP-4 in spinal cord was detected. Results: The expression of AQP-4 was significantly increased in the gray and white matter of spinal cord one day after spinal cord injury, and reached their peak at 3 day in both two groups. Meanwhile, that in the injury group was more obvious(P<0.05). The increase of AQP-4 expression in the Chinese traditional medicine group were parallel with the improvement of nerve function at the 7th,14th and 21st day. As compared with injury group, the expression of AQP-4 in the Chinese traditional medicine group was significantly decreased(P<0.01). Conclusion: By inhibiting the expression of AQP-4 after SCI, Buyanghuanwu Tang formula can eliminate edema of spinal cord to alleviate successive SCI, so as to protect the residual spinal cord tissues and to promote recovery of spinal cord function.
出处
《中国康复》
2005年第1期3-5,共3页
Chinese Journal of Rehabilitation
基金
国家自然科学基金课题资助(30371837)
