褐藻酸降解酶的制备及其性质研究

Preparation of alginase and its properties

通过培养褐藻酸降解菌交替单胞菌(Alteromonassp.)菌株H -1使其产酶,研究了该酶的性质。结果表明 ,该菌在25℃培养72h时产酶量最高。褐藻酸酶作用的最适底物质量分数为1%～2% ,最适pH值为7.5 ,最适反应温度为40℃ ,温度升高酶活力急剧下降。

Alginase was prepared by culturing an alginic acid decomposing bacterium Alteromonas sp.strain H-1.The bacterium produced a maximum amount of alginase when it was incubated at25℃for72h.The optimum alginate sodium concentration was1%～2%,the optimum pH and temperature was7.5(Tris-HCl buffer)and40℃respec-tively.The activity of the enzyme would reduce sharply if the temperature was above40℃.