摘要
通过培养褐藻酸降解菌交替单胞菌(Alteromonassp.)菌株H -1使其产酶,研究了该酶的性质。结果表明 ,该菌在25℃培养72h时产酶量最高。褐藻酸酶作用的最适底物质量分数为1%~2% ,最适pH值为7.5 ,最适反应温度为40℃ ,温度升高酶活力急剧下降。
Alginase was prepared by culturing an alginic acid decomposing bacterium Alteromonas sp.strain H-1.The bacterium produced a maximum amount of alginase when it was incubated at25℃for72h.The optimum alginate sodium concentration was1%~2%,the optimum pH and temperature was7.5(Tris-HCl buffer)and40℃respec-tively.The activity of the enzyme would reduce sharply if the temperature was above40℃.
出处
《海洋科学》
CAS
CSCD
北大核心
2005年第2期78-80,共3页
Marine Sciences
基金
国家海洋863资助项目 (D12022509
D10022508)
关键词
褐藻酸降解菌
褐藻酸降解酶
制备
alginic acid decomposing bacterium
alginase
preparation
property