水稻sbe1启动子驱动的反义sbe-GUS融合基因在转基因水稻中的表达

Expression of the Antisense-sbe-GUS Fusion Genes Driven by the Rice sbe1 Promoter in Transgenic Rice

为研究水稻基因启动子对外源基因在转基因水稻中表达的影响 ,构建了由sbe1启动子引导的反义sbe GUS融合基因。经农杆菌介导 ,将不同的融合基因导入水稻中 ,定量测定转基因水稻植株不同组织中的GUS酶活力。结果表明 ,sbe1启动子可驱动反义sbe GUS融合基因在转基因水稻植株的胚乳中高效表达 ,而在颖壳、胚和茎叶等组织中的表达活性较弱。证实sbe1启动子在驱动外源基因的表达上表现有明显的组织特异性。

To confirm the effect of the rice sbe 1 promoter on foreign gene expression in transgenic rice plants, three antisense- sbe - GUS fusion genes were constructed and then fused into the upstream region of the rice sbe 1 promoter (Fig.2). All of these constructs were introduced into rice via Agrobacterium -mediated transformation, and the integration of the T-DNAs was confirmed by PCR analysis (Fig.3). GUS expression showed that the GUS(beta-glucuronidase) activity was strongest in the endosperm of transgenic rice plants, while very weak or no activity could be detected in other tissues such as hull, embryos, leaf and stem. It indicated that all of anti- sbe - GUS fusion genes could be tissue-specifically expressed in transgenic rice when driven by the rice sbe 1 promoter (Fig.4, Table 1).