酶免疫法检测儿童粪便幽门螺杆菌抗原63例

Enzyme immunoassay for detecting helicobacter pylori stool antigen in feces

目的探讨酶免疫法检测儿童幽门螺杆菌粪便抗原 (HpSA) 在诊断幽门螺杆菌 (Hp) 感染中的价值。方法用酶免疫法检测63例胃部不适患者粪便标本 HpSA, 并同时进行传统的胃镜组织学法和快速尿素酶试验 (RUT)。通过两者相比较, 对酶免疫测定法进行初步评价。结果酶免疫测定法的敏感性为 89. 5%, 特异性为83. 3%, 准确性为96 8%, 阳性预测值为94. 4%, 阴性预测值为91. 1%。结论酶免疫测定法检测HpSA具有较高的敏感性和特异性, 而且方法简便, 取材方便, 并适合流行病学调查, 是一项值得推广的临床检测方法。

Objective To evaluate the clinical utility for the diagnosis of helicobacter pylori(Hp)infection with enzyme immunoassay(EIA)for detecting children Hp stool antigen(HpSA)in feces.Methods HpSA in stool specimens of 63 patients were detected by EIA.All of the patients also underwent gastroscopy histologic methods and rapid urease test(RUT).The results of the HpSA detected with EIA was compared with that detected with gastroscopy,and the EIA was evaluated.Results The sensitivity and specificity of EIA were 89.5% and 83.3% respectively,the positive and negative predictive values were 94.4% and 91.1% respectively,and the accuracy was 96.8%.Conclusion EIA for detecting HpSA in feces is a rapid,noninvasive method,it both has high sensitivity and high specificity;it suits epidemiology inquisition,and is a clinical examination method worthy of being expanded.