体外培养海马神经干细胞分化前后离子通道检测

Detection of ion channels of in vitro cultured neural stem cells before and after the differentiation in seahorse

目的:研究神经干细胞(NSCs)分化前后电压依赖性Na+、K+、Ca2+通道的变化。方法:应用全细胞膜片钳技术检测NSCs分化前及分化后3d、7d、14d、21d电压依赖性Na+电流(INa)、延迟整流性K+电流(IK)、电压依赖性Ca2+电流(ICa)。结果: 分化前后均未检出INa,分化前未检出IK,分化后3d、7d、14d、21d,IK检出率分别为11.76%、24.75%、46.51%、76.18%,ICa在分化前后均可检出,分化前和分化后各时间点ICa的幅度分别为(119±73)pA、(213±98)pA、(324±151)pA、(735±312)pA、(1079±307)pA。结论:分化前后均未检出INa,随分化时间延长,IK检出率逐渐增加,ICa幅度逐渐增高。

objective: To study the change of voltage dependent Na+, K+and Ca2+channels before and after the differentiation of neural stem cells. Methods:Whole-cell recording technique was used to detect voltage dependent Na+current, Delayed rectifier K+current, voltage dependent Ca2+current at 3d,7d,14d and 21d before and after the NSCs differentiation. Results: INa failed to be detected before and after the NSCs differentiation. The percent of IKdetected was 11. 76%, 24. 75%, 46. 51% and 76. 18%, respectively at 3d, 7d, 14d and 21d after differentiation. The amplitude of ICa at any time recorded was (119 ± 73)pA, (213±98)pA,(324±151)pA,(735±312)pA and 1079±307)pA before and after the differentiation. Conclusion: INa failed to be detected before and after the differentiation. Both the percent of IKdetected and the amplitude of IN. increases when the time of differentiation became longer.