传统肝硬化动物模型制备方法的改良

Improvement on the traditional way of building liver cirrhosis animal model

目的:探索传统肝硬化大白鼠模型制备方法的改良的可能性及可行性。方法:取168只Wistar大白鼠,对56只大白鼠使用传统模型制备方法建立肝硬化模型,对56只大白鼠使用复合病因法建立肝硬化模型,对56只大白鼠使用改良模型制备方法建立肝硬化模型。结果:传统的方法进行肝硬化门脉高压模型的制造需要60d,大鼠死亡率为53.6%,复合因素制备方法需要42d,大鼠死亡率为41%;改良方法制备需要66d,大鼠死亡率为23.2%。结论:肝硬化模型的改良方法具有降低大白鼠死亡率、节约经费等特点。

Objective: To discuss the possibility and feasibility of the improvement on the traditional way of building the liver cirrhosis r.at model. Methods: We selected 168 Wistar rats, and used 56 rats to build the liver cirrhosis models in the traditional way , 56 rats to build the liver cirrhosis models in the complex pathogenesis way and 56 rats to build the liver cirrhosis models by the improvement method. Results: We needed 60d to build the live cirrhosis model in the traditional way, with the death rate of 53. 6%, 42d in the complex pathogenesis way, with the death rate of 41%, and 66d by the improvement way, with the death rate of 23. 3%. Conclusion: The improvement method of preparing the liver cirrhosis model can save money, with lower rat death rate.