摘要
目的:研究染料木黄酮(genistein,Gen)对慢性缺氧性肺动脉高压(HPH)的预防作用,为防治HPH提供一种新的途径。方法:健康雄性Wistar大鼠,体重(210.3±28.7)g,随机分为5组:①平原对照组(C);②慢性缺氧组(H);③缺氧+Gen低剂量组(H+L):25mg/kg bw;④缺氧+Gen中剂量组(H+M):50mg/kg bw;⑤缺氧+Gen高剂量组(H+H):100mg/kg bw;C组在平原,②～⑤组每天灌胃大鼠受试物或与受试物等容积的二甲基亚砜(DMSO),置于减压舱,模拟海拔5000m高原,8h/d,持续21d,分别测定肺动脉压、右心室功能和右心室肥大指数。结果:Gen可以显著抑制慢性缺氧引起的肺动脉压升高(P<0.01)。单纯缺氧组右心室肥大指数RV/(LV+Sep)显著增加(P<0.01);与单纯缺氧组比较,Gen各组RV/(LV+Sep)从低剂量到高剂量呈逐渐降低的趋势(40.53±3.80)%,(39.07±3.69)%,(33.73±3.20)%,但仅高剂量组(100mg/kg bw)有显著性差异(P<0.01)。结论:Gen可以有效防治慢性缺氧性肺动脉高压,对慢性缺氧诱导的右心室肥大的预防作用与剂量有关,其作用机制有待进一步探讨。
Objective: To study the preventive effects of genistein (Gen) on chronic hypoxic pulmonary hypertension (HPH). Methods: Adult male Wistar rats were randomly divided into 5 groups: ①Control group (C); ②Hypoxic group(H); ③Gen low dose group (25mg/kg bw, H+L); ④Gen medium dose group (50 mg/kg bw, H+M); ⑤Gen high dose group (100 mg/kg bw, H+H) . ②~⑤ groups were exposed to 21 d of hypobaric hypoxia daily (simulate altitude 5 000m) for 8 h everyday. During their exposure to hypoxia, rats were i.g. administered with solvent (DMSO) or Gen. The pulmonary artery blood pressure (PAP) ,cardiac function of right ventricle and index of right ventricular hypertrophy were assessed. Results: PAP was significantly greater in chronic hypoxic rats than that of the control group (P<0.01). and significantly lower in three Gen groups than that in hypoxic group, and the dosage of 50 mg/kg bw was themost effective (F<0.01). The chronic hypoxic rats had a markedly greater ventricular hypertrophy (RV/LV+Sep) compared with normoxic rats. RV/LV+Sep (%) in Gen group diminished from lower to higher dosage 40.53±3.80, 39.07±3.69, 33.73±3.20. but only significant at 100mg/kg bw as compared to the chronic hypoxic group (P<0.01). Conclusions: Gen could significantly reduce and inhibit the development of HPH, and administration of Gen could inhibit right ventricular hypertrophy in a dose dependent manner.
出处
《营养学报》
CAS
CSCD
北大核心
2005年第1期42-45,共4页
Acta Nutrimenta Sinica
