微核直径测试作为非整倍体诱发剂的分析手段

Measurement of Micronucleus Diameters as a Test for the Detection of Aneugens

以小鼠骨髓红细胞微核直径测试,比较了秋水仙素(COL)与昆明山海棠(THH)、对苯二酚(HQ)在哺乳动物体细胞中的非整倍体诱发效应,丝裂霉素C(MMC)作为多功能染色体断裂剂引入实验,为诱发非整倍体的阴性对照,结果发现,COL组,71％的微核直径(d)大于或等于所在细胞直径(D)的五分之一(d≥D/5);THH诱发微核中,54％的微核d≥D/5;HQ及MMC组,分别有47％及14％的微核相对直径达此阈限,暗示THH及HQ具有类似COL的某种非整倍体诱发效应。微核直径测试可作为非整倍体诱发剂检测的辅助手段。

The relative diameters of micronucleus induced by colchicine(COL),Triptergium hypoglaucum(Level) Hutch(THH) and hydroquinone(HQ) were compared to evaluate their aneugenic activities in mouse bone marrow erythrocytes.Mitomycin C(MMC) was taken as the negative control in the experiment because it is a multifunctional clastogen without aneugenic potential.Diameters of the cytoplasm(D) and the micronucleus(d) of each micronucleated erythrocytes were measured with a micrometer in a microscope.The frequency of relatively large micronuclei(d>D/5) was found(71%) in COL treated group.In the THH and HQ treated groups,the relatively large micronuclei were 54% and 47%,respectively.Such micronuclei were infrequent(14%) in the MMC treated group.The results implied that THH and HQ may possess some aneugenic potential like COL.