摘要
以卡尼鄂拉蜂 (Apismelliferacarnica)和高加索蜂 (Apismelliferacaucasica)的雄蜂精液对意大利蜜蜂(Apismelliferaligustica)的处女蜂王进行人工授精 ,并利用 11个引物进行随机扩增多态DNA(RAPD)标记分析。根据RAPD标记计算工蜂个体间的平均遗传相似系数 ,然后计算蜂王与雄蜂交配的数量。结果表明 ,RAPD标记是测定蜂王与雄蜂交配数量的一种有效方法。
Virgin queen of Apis mellifera ligustica was artificially inseminated with semen from drones of Apis mellifera carnica and Apis mellifera caucasia. Eleven primers were used for radom amplified polymorphic DNA analysis. The number of drones mated with the queen was calculated based on the average genetic similar coefficient between drone individuals according to RAPD analysis. The results indicated that RAPD analysis is a effective method in determining the number of drone mated with a queen.
出处
《经济动物学报》
CAS
2003年第4期42-45,共4页
Journal of Economic Animal
基金
国家自然科学基金资助项目 ( 3 0 160 0 68)
