摘要
目的:探讨去神经后大鼠骨小梁结构的变化及干预对骨质疏松后骨折的预防具有重要的意义。方法:选择10周龄SD雄性大鼠行双侧后肢坐骨神经和股神经离断术后,采用石膏固定,被动运动等干预手段30d后取胫骨近端1/3,用计算机图像处理分析系统计算骨小梁体积密度、骨小梁平均宽度、骨小梁连接点数、游离末端数。结果:正常组大鼠骨小梁体积密度为(44.80±7.68)%,骨小梁平均宽度为(46.4±3.6)μm,骨小梁连接点数为(14.28±2.70)个/mm2,游离末端数为(12.86±2.94)个/mm2,去神经组大鼠分别为(24.30±8.26)%,(21.2±3.4)μm,(2.17±0.81)个/mm2,(18.97±2.44)个/mm2,去神经后固定组分别是(17.30±6.38)%,(15.2±9.3)μm,(0.96±0.43)个/mm2,(22.97±5.31)个/mm2,而去神经被动运动组则分别是(26.8±12.3)%,(23.6±1.7)μm,(7.18±2.30)个/mm2,(17.40±2.47)个/mm2。与正常组比较,失神经大鼠骨小梁体积密度,骨小梁平均宽度,骨小梁连接点数显著减少,游离末端数显著增加,尤其是失神经后固定组。结论:去神经后骨组织因失去了应力作用而发生骨小梁结构破坏,去神经后固定加重了这种趋势,而被动运动可逆转这种趋势。
AIM:To study the significance of changes of trabecular bone microarchitecture in denervated rats and interventions in the prevention of fracture after osteoprosis.METHODS:After the sciatic nerve and femoral nerve of hindlimbs in 10 week old SD rats were denervated,the hindlimbs were fixed with plaster,and passively moved for 30 days.Computer image processing and analyzing system was operated to calculate the bulk density,average width,connection sites and dissociated ends of the trabecular bone of 1/3 proximal tibia.RESULTS:The bulk density,average width,connection sites and dissociated ends of trabecular bone were (44.80±7.68)%,(46.4±3.6) μm,(14.28±2.70) sites/mm2 and (12.86±2.94) ends/mm2 respectively in control group,(24.30±8.26)%,(21.2±3.4) μm,(2.17±0.81) sites/mm2,(18.97±2.44) ends/mm2 respectively in denervation group,(17.30±6.38)%,(15.2±9.3) μm,(0.96±0.43) sites/mm2,(22.97±5.31) ends/mm2 respectively in fixation after denervation group,and (26.8±12.3)%,(23.6±1.7) μm,(7.18±2.30) sites/mm2,(17.40±2.47) ends/mm2 respectively in passive movement after denervation group.Compared with the control group,the bulk density,average width and connection sites of trabecular bone were obviously decreased,and dissociated ends were significantly increased in the denervation groups,especially in the fixation after denervation group.CONCLUSION:The trabecular bone microarchitecture of denervated bones will be damaged due to the loss of stress,and fixation after denervation deteriorates such tendency,but passive movement ameliorates it.
出处
《中国临床康复》
CSCD
北大核心
2005年第4期182-183,共2页
Chinese Journal of Clinical Rehabilitation