水稻脂质氧化酶同工酶种质储藏特性的研究

Study on the Difference of the Durable Storage Character of Rice Grain with the Loss of Lipoxygenase Isoenzymes

对水稻脂质氧化酶(LOX)同工酶缺失的单体及聚合体人工老化实验研究表明:不同LOX的缺失突变体之间,以LOX-1,LOX-2、LOX-3全缺的D1308、D1311的发芽率始终最高,老化指数最低;LOX-1缺失所造成的影响要大于LOX-2缺失和LOX-3缺失。LOX缺失的单体及聚合体具有较好的储藏特性,LOX的缺失对保持种子生活力和延缓稻谷陈化变质具有重要的作用。进一步的分析认为LOX-1的缺失阻断或延缓了LOX-2和LOX-3所启动的脂质过氧化反应,从而延缓了稻谷的陈化变质,减轻种子生活力的下降。LOX-1可能是控制稻谷陈化变质和种子衰老的关键基因。

It is shown through accelerated aging tests that among all materials D1308, D1311 with null- LOX-1, LOX-2 and LOX-3 have the highest germination ratio and the lowest aging index ;wanjian2090 and D1305 with null- LOX-1?LOX-2 are more storageble than those with null- LOX-2, LOX-3 and those with null- LOX-2 or null- LOX-3; D1304, D1312 with null- LOX-2, LOX-3 are not apparently more storageble than those with null- LOX-2 or null- LOX-3.It indicates the effects of null- LOX-1 are more profound than those of null- LOX-2 or null- LOX-3. The materials lacking single or compound lipoxygenase have much better storage character than those common ones .The lacking of lipoxygenase can stave the rice from deteriation effectively and the lacking of LOX-1 cut off or stave the lipid superoxidation which are triggered by LOX-2 and LOX-3.Above all LOX-1 is probably the key gene responsible for the long life span of rice grains.