山新杨组织培养快繁技术研究

Study on Rapid Multiplication Techniques by Tissue Culture in Shanxinyang(P.davidiana X P.bollena )

山新杨冬季枝条经FC处理,选择萌发新枝的茎段和叶片为接种外植体;诱导茎段产生腋芽的培养基为1/2MS+6-BA0.2～0.5mg/L+NAA0～0.5mg/L,诱导叶片产生不定芽的培养基为1/2MS或MS+6-BA0.5mg/L+NAA0.5mg/L;丛生芽快繁培养基MS+6-BA0.3g/L+NAA0.3mg/L,每20天继代一次,繁殖倍数20左右;生根培养基为1/2MS或MS,附加0.1～0.5mg/L的多效唑,对生根有促进作用.

Stem segments and leaves of new shooting, which are from winter shoot treated by FC , was used as inoculating explant. The medium used for producing axillary bud from stem segment is 1/2MS+ 6-BA 0.2~0.5 mg/L + NAA 0~0.5mg/L; the medium used for generating adventitious bud is 1/2MS or MS + 6-BA 0.5mg/L + NAA0.5mg/L; medium used for producing rosette buds is MS+6-BA0.3mg/L + NAA0.3mg/L. Regeneration was conducted every 20 days and 20 times of multiplication could be achieved. Rooting medium is 1/2MS or MS. Adding benzimidazole 0.1~0.5mg/L in the medium could promote rooting.